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The primers sequences of target genes and reference gene
| Gene | Sequence (5′–3′) |
|---|---|
| Caspase-1 | |
| Forward | AGTGCAGGACAACCCAGCTATG |
| Reverse | CAAGACGTGTGCGGCTTGA |
| GSDMD | |
| Forward | TGAATGTGTACTCGCTGAGTGTGG |
| Reverse | CAGCTGCTGCAGGACTTTGTG |
| GAPDH | |
| Forward | TCTACTGGTTCAGCAGCCATCTTTA |
| Reverse | TGGTGAAGACGCCAGTGGA |
GSDMD gasdermin D, GADPH glyceraldehyde-3-phosphate dehydrogenase
