Table 2.

RT-qPCR primers used to test the expression of kidney renin as a dehydration marker and vocal fold genes selected based on differentially regulated proteins

Gene symbol	Protein name	mRNA NCBI Ref Seq	Primers 5′ - 3’		Product size (bp)
Kidney genes
REN	Renin	XM_017347610.1	Fw	GACATAGTGACTGTGGGCGG	87
			Rv	CTTGGCCAGCATGAAGGGTA
GAPDH*	Glyceraldehyde-3-phosphate dehydrogenase	NM_001082253.1	Fw	GTCGGAGTGAACGGATTTGG	81
			Rv	GGCGACAACATCCACTTTGC
Vocal fold genes
LAMC1	Laminin subunit gamma 1	XM_017339901.1	Fw	CCTTCGGAGACGAGGTGTTC	95
			Rv	GTTGCACTTGCACCTGCCG
FBLN5	Fibulin 5	XM_002719596.3	Fw	GCTCTCTGCTCCCAACTACC	101
			Rv	CACACTCGTCCACATCCACA
COL18A1	Collagen XVIII alpha-1chain/ Endostatin domain-containing protein	XM_008253234.2	Fw	GGTCAACCTCAAGGACGAGG	198
			Rv	GTCTCGCAGTAGCTGTTGGT
MFAP4	Microfibril associated protein 4	XM_002722656.3	Fw	GGCAAGTGGACGGTTTTCC	71
			Rv	TTGTAGTCATTCCAGCCCCG
FBN1	Fibrillin 1	XM_008269057.2	Fw	TCAATGGCTACCCCAAACGG	78
			Rv	CAGACTGATCCTCAACAGTGG
MFAP5	Microfibril associated protein 5	XM_002712855.3	Fw	CAGGGAGTGGCTTTGTTCCT	182
			Rv	CCAAGCGCATCCATTCAGC
MYL2	Myosin regulatory light chain 2	XM_002719750.3	Fw	GGGAGAAACTCAAGGGAGCG	95
			Rv	GTAGTCGGCCTTCAGCACTC
DMD	Dystrophin	XM_017349731.1	Fw	TGCGGTTCAGAAGCAAAACG	170
			Rv	TCTCATCAGGAGGCAGCTCT
SLMAP	Sarcolemmal membrane-associated protein	NM_001082348.2	Fw	GCACAGTCAGAAACTGAGGC	196
			Rv	TCTGCAGTTGGGCTTGAAGA
LMNA	Lamin A/C	XM_008264325.1	Fw	CAAGACCCTCGACTCTGTGG	102
			Rv	TTCTTGGTGTTGCGCGCTTT
NIF3L1	NIF3-like protein 1	XM_002712443.3	Fw	TCGGAGCCTGTACATCCAGA	180
			Rv	CCTCACCATCAGTCCTAGCAG
HPRT1*	Hypoxanthine phosphoribosyltransferase 1	NM_001105671.1	Fw	GATGGTCAAGGTCGCAAGCC	73
			Rv	TCCAACAAAGTCTGGCCTGT

*normalizer genes used for relative expression calculation of target genes