Fig. 5.

β₃-adrenoceptor function in gracilis PVAT is lost in obesity, and the vasodilator function of adiponectin is lost. a Gracilis arteries from obese mice with and without PVAT were subjected to EFS, before incubating with CL-316,243 for 30 min and repeating the stimulus (n = 7, p > 0.05 all comparisons). b Following the control EFS protocol, PVAT-denuded vessels were allowed 15 min to recover before incubating with recombinant globular mouse adiponectin (5 μg/mL) and repeating the stimulus (n = 4, p > 0.05). Data shown are mean ± SEM (−PVAT vs. +PVAT vessels were tested using two-way ANOVA. Before and after drugs within the same vessel type, e.g., −PVAT before drug vs. −PVAT after drug were tested using repeated-measures ANOVA. Both followed by Bonferroni post hoc tests).