Fig. 8.

Activation of NOS restores PVAT function in obesity in mesenteric arteries but not in the gracilis artery. −PVAT mesenteric (a) and gracilis (b) arteries from obese mice with a section of exogenous PVAT suspended above were stimulated with EFS. The exogenous PVAT was removed and incubated for 30 min with histamine (100 μM), before returning the PVAT to the organ bath and repeating the stimulus (n = 8, a −PVAT vs. exogenous PVAT p > 0.05, exogenous PVAT vs. histamine-treated PVAT p < 0.001***, −PVAT vs. histamine-treated PVAT p < 0.05⁺. b p > 0.5 all comparisons). Data shown are mean ± SEM (−PVAT vs. +PVAT vessels were tested using two-way ANOVA. Before and after drugs within the same vessel type, e.g., +exogenous PVAT before drug vs. +exogenous PVAT after drug were tested using repeated-measures ANOVA. Both followed by Bonferroni post hoc tests).