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Dose response curve for recombinant rSP-D binding to A. fumigatus conidia. Aliquots of 2 × 106 conidia were incubated with various concentrations of SP-D for 1 h at 25°C followed by washing and similar incubations with primary and secondary antibodies. Binding was detected by flow cytometry and normalized to the mean fluorescent intensity at 20 μg/ml. Data are the averages of duplicate analysis.
