FIG. 7.

Inhibition of AP hSP-A and AP hSP-D binding to A. fumigatus conidia by rat HSC. Purified AP hSP-A or AP-hSP-D (20 μg/ml) was incubated with various concentrations of rat HSC (based on total phospholipid content) for 15 min at 25°C. The surfactant protein-inhibitor mixture was then added to 2 × 10⁶ conidia and binding was allowed to progress for 1 h at 25°C, followed by washing and similar incubations with primary and secondary antibodies. Binding was detected by flow cytometry and normalized to the mean fluorescent intensity in the absence of inhibitor. The data are the averages ± standard errors of three independent experiments. ∗, P < 0.05 compared to binding in the absence of HSC.