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. 2022 Sep 7;21(23):2484–2498. doi: 10.1080/15384101.2022.2103339

Figure 1.

Figure 1.

CircPLOD2 was increased and associated with the poor prognosis of colon cancer. (A) qRT-PCR analysis of circPLOD2 between colon cancer and related normal colon tissues (n = 10 per group). ***p < 0.001 vs. the tumor group. (B) Relative expression of circPLOD2 in colon cancer and normal colon cell lines. **p < 0.01, ***p < 0.001 vs. the RCM-1 group. (C) The expression level of circPLOD2, U6 and GAPDH located in the cytoplasm and nuclear of HCT-116 and SW620 cells were determined by nuclear-cytoplasmic fractionation assay. (D) Representative image of the gel electrophoresis was performed for the existence of circPLOD2 in HCT-116 cells. GAPDH was used as the linear control. (E) Relative expression of linear PLOD2 and circPLOD2 in HCT-116 and SW620 cells with or without RNase R treatment was measured by qRT-PCR. (F) The overall survival rate of the patients with high or low circPLOD2 expression was analyzed using the Kaplan-Meier method. N = 3, all data were represented as mean ± SD.