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. 2022 Jul 25;21(23):2471–2483. doi: 10.1080/15384101.2022.2102796

Figure 4.

Figure 4.

Circ_0057452 regulated the proliferation, cell cycle, migration, invasion, apoptosis, and collagen synthesis of KFs by sponging miR-7-5p. (a) The expression of miR-7-5p was examined by qRT-PCR in KFs transfected with anti-miR-NC or anti-miR-7-5p. (b-i) KFs were transfected with si-NC, si-circ_0057452#1, si-circ_0057452#1 + anti-miR-NC, or si-circ_0057452#1 + anti-miR-7-5p. (b and c) MTT assay and Edu assay were utilized to evaluate proliferation ability. (d and e) Cell cycle distribution and cell apoptosis were detected by flow cytometry analysis. (f) The protein levels of Bax, Bcl-2 and cleaved-caspase-3 were analyzed by western blot assay. (g) Cell migration was evaluated by wound healing assay (×40). (h and i) Transwell assay was used to detect cell migration and invasion (×100). (j) The protein levels of fibronectin, collagen I and collagen III were examined by western blot assay. *P < 0.05.