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. 2022 Jul 25;21(23):2471–2483. doi: 10.1080/15384101.2022.2102796

Figure 5.

Figure 5.

GAB1 was targeted by miR-7-5p. (a) The putative binding sites between miR-7-5p and GAB1 were predicted by starBase. (b-d) The interaction between miR-7-5p and GAB1 was confirmed by dual-luciferase reporter, RNA pull-down, and RIP assays. (e and f) The mRNA and protein expression of GAB1 in KFs transfected with miR-NC or miR-7-5p were determined by qRT-PCR and western blot, respectively. (g and h) GAB1 mRNA and protein expression in normal skin tissues and keloid tissues were detected by qRT-PCR and western blot, respectively. (i and j) The mRNA and protein levels of GAB1 in NFs and KFs were examined by qRT-PCR and western blot, respectively. (k and l) QRT-PCR and western blot were conducted to detect the mRNA and protein levels of GAB1 in KFs transfected with si-NC, si-circ_0057452#1, si-circ_0057452#1 + anti-miR-NC, or si-circ_0057452#1 + anti-miR-7-5p. *P < 0.05.