FIG. 2.

Cytokine responses of splenocytes of immunized mice after a booster injection with p38 in IFA or alum. Three mice per group were immunized s.c. or i.p. with 3 μg of p38 in IFA or alum and boosted 14 days later by the same route. Fourteen days after the second injection, the splenocytes were prepared and assayed for cytokine production in response to p38 (5 μg/ml; A) or P4 (1 μg/ml; B) as described in Materials and Methods. Cytokine levels are expressed as net mean ± SD of triplicate cultures after subtraction of the mean for medium only. Data are representative of three separate experiments with similar results.