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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: Adv Nanobiomed Res. 2022 Aug 21;2(10):2200030. doi: 10.1002/anbr.202200030

Figure 1.

Figure 1.

Fabrication methods of granular hydrogels. (A) Batch water-in-oil emulsion fabrication of PEG-MAL granular hydrogels. 4-arm PEG-MAL macromer and 4-arm PEG-SH crosslinker at pH 5.4 is added into a mineral oil and surfactant solution and quickly vortexed for 30 seconds while crosslinking occurs. Microspheres are isolated using centrifugation and washed to remove excess oils. (B) Mechanical fragmentation of bulk 4-arm PEG-MAL hydrogels. The bulk gel is minced, added to a volume of DI Water, and fragmented for 4 minutes using a 20 mm tissue homogenizer. Fragmented gels are isolated by centrifugation.