| Genetic reagent (Drosophila melanogaster) |
Rho172R
|
|
|
Strutt et al., 1997
|
| Genetic reagent (Drosophila melanogaster) |
y[1]w[*];Mi{y[+mDint2]=MIC}cv-c[MI00245] |
BDSC |
RRID:BDSC_30677
|
|
| Genetic reagent (Drosophila melanogaster) |
P{Sxl-Pe-EGFP.G}G5b, w* |
BDSC |
RRID:BDSC_32565
|
|
| Genetic reagent (Drosophila melanogaster) |
w1118; P{10XStat92E-GFP}1
|
BDSC |
RRID:BDSC_26197
|
Bach et al., 2007
|
| Genetic reagent (Drosophila melanogaster) |
cv-cM62
|
|
|
Denholm et al., 2005
|
| Genetic reagent (Drosophila melanogaster) |
cv-c7
|
|
|
Denholm et al., 2005
|
| Genetic reagent (Drosophila melanogaster) |
cv-cC524
|
|
|
Denholm et al., 2005
|
| Genetic reagent (Drosophila melanogaster) |
UAS-cv-cWT
|
|
|
Sotillos et al., 2018
|
| Genetic reagent (Drosophila melanogaster) |
UAS-cv-cGAPmut
|
|
|
Sotillos et al., 2018
|
| Genetic reagent (Drosophila melanogaster) |
UAS-cv-cΔStART
|
|
|
Sotillos et al., 2018
|
| Genetic reagent (Drosophila melanogaster) |
UAS-Myc-DLC3WT
|
|
|
Sotillos et al., 2018
|
| Genetic reagent (Drosophila melanogaster) |
nos-nod::GFP
|
A. González Reyes |
|
|
| Genetic reagent (Drosophila melanogaster) |
c587-Gal4
|
E. Matunis |
|
RRID:BDSC_67747
|
| Genetic reagent (Drosophila melanogaster) |
six4-moe::GFP
|
S. DiNardo |
|
Sano et al., 2012
|
| Genetic reagent (Drosophila melanogaster) |
Vasa::GFP
|
P. Lasko |
|
|
| Genetic reagent (Drosophila melanogaster) |
Perlecan::GFP
|
|
|
Morin et al., 2001
|
| Genetic reagent (Drosophila melanogaster) |
twist-G4
|
|
|
Greig and Akam, 1993
|
| Genetic reagent (Drosophila melanogaster) |
UAS-Myc-DLC3S993N
|
This paper |
|
Methods |
| Recombinant DNA reagent |
XhoI DLC3WT::pBS |
This paper |
|
Methods |
| Recombinant DNA reagent |
XhoI DLC3 S993N::pBS |
This paper |
|
Methods |
| Recombinant DNA reagent |
pUASt::Myc-DLC3S993N
|
This paper |
|
Methods |
| Sequence-based reagent |
DLC3S993N-For |
This paper (Sigma-Aldrich) |
PCR primers |
5’-TGTACCACTATGTCACCGACA-A-CATGGCACC-3’ |
| Sequence-based reagent |
DLC3S993N-Rev |
This paper (Sigma-Aldrich) Methods |
PCR primers |
5’-TGGGGTGCCATG-T-TGTCGGTGACATAGTG-3’ |
| Antibody |
Rat monoclonal anti-VASA |
DSHB |
AB_760351 |
(1:20) |
| Antibody |
Mouse monoclonal anti-Nrt |
DSHB |
AB_528404 |
(1:100) |
| Antibody |
rat monoclonal anti-DE-cad |
DSHB |
AB_528120 |
(1:50) |
| Antibody |
Rabbit polyclonal anti-STAT |
E. Bach |
Flaherty et al., 2010
|
(1:500) |
| Antibody |
Mouse monoclonal anti-β-catenin |
DSHB |
AB_528089 |
(1:100) |
| Antibody |
mouse monoclonal anti-Eya |
DSHB |
AB_528232 |
(1:100) |
| Antibody |
guinea pig polyclonal anti-Ems |
U. Walldorf |
Walldorf and Gehring, 1992
|
(1:5.000) |
| Antibody |
Rabbit polyclonal anti-Sox100B |
S. Russell |
Nanda et al., 2009
|
(1:1000) |
| Antibody |
guinea pig polyclonal anti-Tj |
A. González-Reyes |
Díaz-Torres et al., 2021
|
(1:1000) |
| Antibody |
rabbit polyclonal anti-Perlecan |
A. González-Reyes |
Díaz-Torres et al., 2021
|
(1:1000) |
| Antibody |
Rabbit polyclonal anti-GFP |
Invitrogen |
A11122 |
(1:300) |
| Antibody |
Chicken polyclonal anti-GFP |
Abcam |
ab13970 |
(1:500) |
| Antibody |
mouse anti-βgal |
Promega |
Z378A |
(1:1000) |
| Antibody |
Goat polyclonal anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Invitrogen |
A-11029 |
(1:400) |
| Antibody |
Goat polyclonal anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 |
Invitrogen |
A-21424 |
(1:400) |
| Antibody |
Goat polyclonal anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
Invitrogen |
A-21236 |
(1:400) |
| Antibody |
Goat polyclonal anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Invitrogen |
A-11034 |
(1:400) |
| Antibody |
Goat polyclonal anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 |
Invitrogen |
A-21429 |
(1:400) |
| Antibody |
Goat polyclonal anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
Invitrogen |
A-21245 |
(1:400) |
| Antibody |
Goat polyclonal anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 |
Invitrogen |
A-21435 |
(1:400) |
| Antibody |
Goat polyclonal anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
Invitrogen |
A-21450 |
(1:400) |
| Antibody |
Goat polyclonal anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 |
Invitrogen |
A-48262 |
(1:400) |
| Antibody |
Goat polyclonal anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 |
Invitrogen |
A-48263 |
(1:400) |
| Antibody |
Goat polyclonal anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 |
Invitrogen |
A32931 |
(1:400) |
| Antibody |
Donkey polyclonal anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 |
Invitrogen |
A-21432 |
(1:400) |
| Software |
ImageJ/Fiji |
Fiji |
http://fiji.sc/
|
|
| Software |
AdobePhotoshop/Illustrator |
Adobe |
https://www.adobe.com/
|
|
| Software |
Prism |
GraphPad |
https://www.graphpad.com/data-analysis-resource-center/
|
|
| Software |
Imaris |
Oxford Instruments |
https://imaris.oxinst.com/
|
|
| Software |
SeqBuilder/SeqMan |
DNASTAR |
https://www.dnastar.com/
|
|
| Commercial assay, kit |
DIG RNA Labelling Kit |
Roche |
11 175 025 910 |
|
| Commercial assay, kit |
Qiagen Plasmid Midi Purification Kit |
Qiagen |
12143 |
|
| Other |
Rhodamine Phalloidin (1:100) |
Invitrogen |
R415 |
High-affinity F-actin probe conjugated to red fluorescence dye TRITC |
| Other |
VECTASHIELD Mounting Medium |
Vector Laboratories |
âH-1000 |
Antifade Mounting Medium for preserving fluorescence |
| Other |
DAPI (1:10.000) |
Thermo Fisher Scientific |
62248 |
Blue-fluorescent DNA stain |
| Other |
Pfu polymerase |
Promega |
M774A |
PCR polymerase |
| Other |
DpnI restriction enzyme |
Roche |
10742988001 |
DNA restriction enzyme |
| Other |
Halocarbon oil 27 |
Sigma |
H8773 |
Inert oil to avoid Drosophila embryos/tissues desiccation. |
