Table 1.
A summary of studies addressing changes in microbiota between breast cancer tissue, non-cancerous adjacent tissue and healthy breast tissue.
| REF | Mian methodology | Sample type | Microbiome related results | ||
|---|---|---|---|---|---|
| Normal breast tissue | Non-cancerous adjacent tissues | Breast cancer (BC) | |||
| (80) | Pyrosequencing V4 16S rDNA Pipeline: QIIME |
20 BC patients | ↑Sphingomonas yanoikuyae | ↑Methylobacterium radiotolerans | |
| (81) | V3-V4 16S rRNA sequencing (Illumina) Pipeline: UCLUST |
57 women with invasive breast carcinoma and 21 healthy women | ↑Methylobacterium | ↑Alcaligenacea | |
| (82) | V3-V5 16S rRNA amplified sequencing data | 668 tumor tissues and 72 normal adjacent tissues from The Cancer Genome Atlas (TCGA) | ↑Actinobacteria and Firmicutes | ↑Proteobacteria, Mycobacterium fortuitum and Mycobacterium phlei | |
| (83) | V1-V2 16S rRNA sequencing (Illumina HiSeq) | 22 Chinese patients with benign tumor and 72 malignant BC patients | ↑Propionicimonas, Micrococcaceae, Caulobacteraceae, Rhodobacteraceae, Nocardioidaceae and Methylobacteriaceae (Ethnicity-related); ↓Bacteroidaceae and ↑ Agrococcus (with malignancy) |
||
| (84) | Pathochips array | 20 normal breast tissue and 148 BC tissue | ↑Actinomyces, Aerococcus, Arcanobacterium, Bifidobacterium, Bordetella, Cardiobacterium, Corynebacterium, Eikenella, Fusobacterium, Geobacillus, Helicobacter, Kingella, Orientia, Pasteurella, Peptinophilus, Prevotella, Rothia, Salmonella, and Treponema | ||
| (85) | Pathochips array | 100 women with triple negative BC (TNBC), 17 matched controls and 20 non-matched controls | ↑Arcanobacterium (75%), Brevundimonas, Sphingobacteria, Providencia, Prevotella, Brucella, Eschherichia, Actinomyces, Mobiluncus, Propiniobacteria, Geobacillus, Rothia, Peptinophilus, and Capnocytophaga (Canimorsus) ↑Herpesviridae, Retroviridae, Parapoxviridae, Polyomaviridae, Papillomaviridae (virus) |
||
| (86) | V3 16S-rRNA gene amplicons sequencing (Ion Torrent) | 16 Mediterranean patients with BC | ↓Methylobacterium (↑Ralstonia) | ↑Sphingomonas | |
| (87) | V6 16S rRNA gene sequencing (Illumina MiSeq) Pipeline: QIIME |
58 women after surgery:13 benign, 45 cancerous tumors and 23 healthy women | ↓ Prevotella, Lactococcus, Streptococcus, Corynebacterium and Staphylococcus | ↑Bacillus, Staphylococcus, Enterobacteriaceae (unclassified), Comamondaceae (unclassified) and Bacteroidetes (unclassified) |
|
| (88) | V3-V5 16S rDNA hypervariable taq sequencing (Illumina MiSeq) Pipeline: IM-TORNADO |
28 women undergoing non-mastectomy breast surgery: 13 benign breast disease and 15 invasive BC | ↓Fusobacterium, Atopobium, Gluconacetobacter, Hydrogenophaga and Lactobacillus | ||
| (89) | V4 16S rRNA gene sequencing (Illumina MiSeq) Pipeline: Mothur |
25 women with breast cancer and 23 healthy women | ↓unclassified genus of the Sphingomonadaceae family in NAF | ↑Alistipes | |
| (90) | V4 16S rRNA gene sequencing (Illumina Miseq) | 32 women with BC stage 0 to II | ↓Akkermansia muciniphila (AM) in BC patients with elevated body fat. | ||
| (91) | V3-V4 and V7-V9 16S rRNA gene sequencing | 221 patients with breast cancer, 18 individuals predisposed to breast cancer, and 69 controls. | ↑Stenotrophomonas
and Caulobacter |
↓Propionibacterium and Staphylococcus | |
| (92) | 16s rRNA gene sequencing;Quantitative Insights into Microbial Ecology (QIIME) tool;RStudio | Bilateral normal breast tissue samples (n = 36) and breast tumor samples (n = 10) | ↑(OUT)[Mogibacteriaceae] family, and Flavobacterium, Acinetobacter, and Brevibacillus genera |
↑(OUT) Ruminococcaceae, Rikenellaceae, genera Butyricimonas, Sutterella, and Akkermansia. |
|
| (93) | Kraken2 and Metaphlan3 | breast tumours and normal tissues (from cancer-free women) of 23 individuals (Slovak); 91 samples obtained from SRA database (China) | ↑Proteobacteria (47%), Bacteroidetes, Firmicutes and Actinobacteria (12%)(Slovak women); ↑Proteobacteria (42%), Firmicutes(42%), Actinobacteria (5%), Cyanobacteria (4%) |
↑Acinetobacter, Rhodobacter, Micrococcus, order Corynebacteriales and Priestia megaterium (Slovak patients) ↑Streptomyces, viruses Siphoviridea and Myoviridae (China patient) |
|
| (94) | Illumina MiSeq sequencing | Tumor tissue and normal tissue in 34 women | ↑Actinobacteria, ↓Proteobacteria, |
↑Firmicutes and Alpha-proteobacteria | |
↑ means up, ↓ means down.