FIG. 6.

Parasiticidal activity of macrophages activated by T cells from TolT-immunized mice. T cells isolated by nylon wool nonadherence were cultured with T. cruzi-infected IC-21 macrophages. On days 4 and 7, the number of motile trypomastigotes in the culture supernatants was counted with a hemocytometer. The percentage of inhibition was calculated as 100 × [(1 − number of parasites in immunized cultures)/number of parasites in nonimmunized cultures]. Each bar represents the average values ± standard deviations obtained from triplicate cultures. Each experiment was performed three times.