Figure 3.

Intracellular signaling phosphorylation in colorectal cancer (CRC)-induced muscle wasting depends on the tumor type. A and C: quantification of Western blots for mTOR, RPS6, and 4E-BP1 phosphorylation in HCT116 and C26 model, respectively. B and D: representative image of Western blots. E and F: Pearson product-moment correlations between each factor and muscle mass, respectively. Data are expressed as fold change from controls ± SD. A two-way ANOVA followed by a Tukey’s multiple comparisons test was performed for all direct comparisons between groups. Significant difference from control: *P ≤ 0.05, **P < 0.01, and ***P < 0.001; xenograft vs. metastasis: #P < 0.05, ##P < 0.01, and ###P < 0.001.