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. 2022 Oct 14;323(6):H1057–H1067. doi: 10.1152/ajpheart.00369.2022

Figure 4.

Figure 4.

Aorta protein expression during the estrous cycle; higher pERα expression in the aorta during estrus. Aorta protein expression for estrogen receptor-α (Erα; A), phosphorylated ERα at serine-167 (pERα; B), and pERα:ERα ratio (C), endothelial nitric oxide synthase (eNOS; D), phosphorylated eNOS at serine-1177 (peNOS; E), pENOS:eNOS ratio (F), Akt (G), and phosphorylated Akt at serine-473 (pAkt; H), and pAkt:Akt ratio (I) for mice in proestrus (P), estrus (E), and diestrus (D). Representative blot to the right. n = 2–8/group. *P < 0.05. pAkt and its Gapdh was taken from Supplemental Fig. 6. lanes 1 (P), 3 (D), and 5 (E). A one-way ANOVA with Tukey’s multiple comparison was used. Data are means ± SD.