Table 1.

Sequences and melting temperatures of the ABO rs657152 primers.

SNP	Primer	Primer sequence	Size, bp	Tm	Annealing temp
ABO rs657152 (A/C)	OF	5′-ACTACTTCATCTGTTACTTCTTATCTTAT-3′	—	61.0°C	55.0°C
	OR	5′-GTGTGAAACTCATCAAAACCGTATA-3′	339	61.0°C
	IF	5′-TATCTCGAATAGCTTCTTGAAGCC-3′	255	61.8°C
	IR	5′-TTGCCTCCCACGTTCCT-3′	124	62.0°C

The specific nucleotides at 3′-end of primers are in bold, while the mismatches are underlined. SNP: single nucleotide polymorphism; OF: outer forward primer; OR: outer reverse primer; IF: inner forward primer; IR: inner reverse primer.