Table 4.

Primers and probes specifications for enteroviruses.

♯	Title	Specification
(A) Non-polio human enteroviruses
F1	Forward primer name	NMF1
2	Oligo scale	0.2 μmol
3	No. of couplings	15 bases
4	Sequence	5′ GCC CCT GAA TGC GGC 3′
R1	Reverse primer name	NMR1
2	Oligo scale	0.2 μmol
3	No. of couplings	19 bases
4	Sequence	5′ AAT TGT CAC CAT AAG CAG C 3′
Pr1	Probe name	MP
2	Conc. per amount	100 pmol/μL
3	Molecular weight	9123
4	Reporter dye	FAM
5	Quencher dye	TAMRA
6	No. of couplings	26 bases
7	Sequence	5′ CGG AAC CGA CTA CTT TGG GTG TCC GT 3′
(B) Human parechovirus
F1	Forward primer name	AN353
2	Oligo scale	40 nmol
3	No. of couplings	29 bases
4	Sequence	5′ GAC AAT AGT TTT GAA ATN ACN ATH CCN TA 3′
R1	Reverse primer name	AN355
2	Oligo scale	0.2 µmol
3	No. of couplings	37 bases
4	Sequence	5′ CTC CAA CTA TAA TGC CAT ART GYT TRT ARA ANC CGY K 3′