FIG. 6.

Anticapsular antibody is more effective than human serum in augmenting T-cell responses. Various encapsulated strains of C. neoformans (613, 68, 3501, T145, and 6) were not pretreated (No Tx) or pretreated by being incubated in normal human serum or with 10 μg of anti-CPS antibody per ml for 1 h at 37°C. The organisms were washed and put into culture at 2 × 10⁵/well with PBMC (2 × 10⁵/well) in medium containing heat-inactivated serum. Seven days later, lymphocyte proliferation was assessed by [³H]TdR incorporation. Each symbol represents an individual experiment. Two-way analysis of variance was performed by the Friedman test, and multiple comparisons determined that significant differences were found between the group that was not pretreated and the group that was treated with MAb.