Reagent	Amount (1×)	Amount (11×)
HF Buffer (5×)	10 μL	110 μL
T7 forward primer (10 μM)	1 μL	11 μL
RT handle reverse primer (10 μM)	1 μL	11 μL
dNTPs (10 mM)	1 μL	11 μL
DMSO	1.5 μL	16.5 μL
Oligo pool (0.5 ng/ μL)	1 μL	11 μL
Phusion polymerase	0.25 μL	2.75 μL
ddH2O	34.25 μL	376.25 μL
Total	50 μL	550 μL