| Reagent | Amount |
|---|---|
| Capped RNA/DMSO solution | 8 μL |
| 10× T4 RNA ligase reaction buffer | 3 μL |
| RNase inhibitor | 1 μL |
| Biotinylated cytidine (bis)phosphate | 1 μL |
| PEG 30% | 15 μL |
| T4 RNA ligase | 2 μL |
| Total | 30 μL |
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