| Reagent | Amount |
|---|---|
| 5× First-Strand Buffer | 4 μL |
| DTT (0.1 M) | 1 μL |
| dNTPs (10 mM) | 1 μL |
| RNasin Plus | 1 μL |
| Superscript III enzyme | 1 μL |
| Total | 8 μL |
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