| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial denaturation | 98°C | 30 s | 1 |
| Denature | 98°C | 10 s | Variable |
| Anneal | 58°C | 30 s | |
| Extend | 72°C | 30 s | |
| Final extension | 72°C | 5 min | 1 |
| Hold | 4°C | Indefinitely | |
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