Buffer A + M

Reagent	Final concentration	Amount
HEPES KOH pH 7.4 (1 M)	30 mM	3 mL
Potassium acetate (2 M)	100 mM	5 mL
Magnesium acetate (1 M)	2 mM	200 μL
DTT (1 M)	2 mM	200 μL
PMSF (0.1 M)	0.1 mM	100 μL
Mannitol (17.5%)	8.5%	48.5 mL
ddH2O	N/A	43 mL
Total		100 mL

Add DTT and PMSF immediately before use.

Storage conditions: Buffer without DTT and PMSF may be stored at 4°C for 6 months.