REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
3-dose HCWs Sera	Evans et al.,1 Qu et al.4	N/A
Omicron BA.1-wave Infected Patient Sera	Evans et al.,1 Qu et al.4	N/A
Omicron BA.4/5-wave Infected First Responders and Household Contacts Sera	This paper	N/A
Chemicals, peptides, and recombinant proteins
Polyethylenimine (PEI)	Thermo Fisher Scientific	Cat# BMS1003
Dulbecco’s Modified Eagles Medium (DMEM)	Sigma-Aldrich	Cat#: 11965-092
Fetal Bovine Serum (FBS)	Thermo Fisher Scientific	Cat#: F1051
0.05% Trypsin + 0.53 mM EDTA	Corning	Cat# 25-052-CI
Penicillin-Streptomycin	HyClone	Cat#: SV30010
Immobilon Crescendo Western HRP substrate	Millipore	Cat# WBLUR0500
QIAprep Spin Miniprep Kit	QIAGEN	Cat# 27106
Coelenterazine	GoldBio	Cat#: CZ2.5, CAS: 55779-48-1
Deposited data
NT50 Values and De-identified patient data	SeroNet Coordinating Center, NCI, NIH	N/A
Experimental models: Cell lines
HEK293T	ATCC	Cat#: CRL-11268, RRID: CVCL_1926
HEK293T-ACE2	BEI Resources	Cat#: NR-52511, RRID: CVCL_A7UK
Calu-3	ATCC	RRID: CVCL_0609
Recombinant DNA
pNL4-3-inGluc	David Derse, NIH Maruzov et al.46	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_D614G	GenScript Biotech Zeng et al.47	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2	GenScript Biotech Evans et al.1	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5	GenScript Biotech Qu et al.4	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4.6	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BF.7	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BQ.1	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BQ.1.1	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75	GenScript Biotech Qu et al.3	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75.2	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_K444T	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_N460K	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_N658S	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_R346T_K444T	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_R346T_N460K	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R346T	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_F486S	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_D1199N	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R346T_F486S	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R346T_D1199N	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_F486S_D1199N	This paper	N/A
Software and algorithms
GraphPad Prism	GraphPad Version 9.0.0	www.graphpad.com
SWISS-MODEL	Guex et al.48	https://swissmodel.expasy.org/
PyMOL	Warren DeLano and Sarin Bromberg	https://pymol.org/
Other
Cytation 5 Imaging Reader	BioTek	N/A