Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 30;6(23):5956–5968. doi: 10.1182/bloodadvances.2022007904

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Figure 2. — RBM12 depletion in primary human CD34⁺HSPCs does not impair erythroid maturation. CRISPR/Cas9 depletion of RBM12 in human CD34⁺ HSPCs via 2 independent sgRNAs (n = 3 biological replicates). (B, C, E, and F) Plotted are means ± standard deviation. (A) Western blot analysis with indicated antibodies at day 15 of differentiation. (B) Growth curve displaying total cell number from day 8 to 12 of differentiation. (C) Left: Representative contour plots of CD71 vs CD235ab at day 15 of differentiation. Right: Quantification of CD71_loCD235ab⁺ and CD71_loCD235ab⁺/HbF⁺ cells. ∗P < .05 by Student’s t test. (D) Representative May-Grünwald Giemsa images of HSPCs at day 15 of differentiation. (E) qRT-PCR analysis of globin genes at day 12 of differentiation. Results are normalized to HPRT1. ∗P < .05 by Student’s t test. (F) qRT-PCR analysis of differentiation markers GATA1, BAND3, and ALAS2 at day 12 of differentiation. Results are normalized to HPRT1.