FIG. 4.

Transcripts in transformants of strain Red3/6. Three transformants of the opaque phase of strain Red3/6 that contained plasmid pAPE(2)/ADE (PCK1p-EFG1) (1 to 3) were diluted into SCAA medium (EFG1 expression) (C) and S4D medium (no EFG1 expression) (S). Cultures were grown at 25°C to an OD₆₀₀ of 1, and total RNA of cells was prepared. Twenty micrograms of RNA was analyzed by Northern blotting using the indicated probes. Ethidium bromide-stained rRNA was used as loading control. w, white; o, opaque.