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. 2022 Nov 8;9:1033864. doi: 10.3389/fvets.2022.1033864

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Copyright © 2022 Niu, Li, Guan, Deng, Wang, Li, Zhou, Xu, Chen, Zhai and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimizing the concentrations of TaKaRa Taq and dNTPs. (A) The optimized electrophoresis result of the optimal TaKaRa Taq concentration; lane M is DNA Marker DL1000, lanes 1–7 represent 0.02 U/μL, 0.04 U/μL, 0.06 U/μL, 0.08 U/μL, 0.10 U/μL, 0.12 U/μL, 0.16 U/μL; Lanes 8–11 are positive controls for TGEV, PEDV, SADS-CoV and PDCoV, respectively; and lane 12 is negative control. (B) The electrophoresis result of the optimal dNTPs concentration in multiplex RT-PCR; wherein, lane M is DNA Marker DL1000, and lanes 1–7 represent 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, and 0.40 mM, respectively; lanes 8–11 are positive controls for TGEV, PEDV, SADS-CoV, and PDCoV, respectively; and lane 12 is a negative control.