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. 2022 Nov 8;9:1033864. doi: 10.3389/fvets.2022.1033864

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Copyright © 2022 Niu, Li, Guan, Deng, Wang, Li, Zhou, Xu, Chen, Zhai and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multiplex RT-PCR primers optimization. (A) is the electrophoresis results of TGEV and PDCoV duplex RT-PCR with different primer ratios; lane M is DNA Marker DL1000; Lanes 1–11 are the primer ratios of 1: 1, 1: 2, 1: 3, 1: 4, 2: 1, 2: 3, 3: 1, 3: 2, 3: 4, 4: 1, 4: 3; Lanes 12–13 are the positive controls for TGEV and PDCoV; and lane 14 is the negative control. (B) The electrophoresis results of PEDV and PDCoV duplex RT-PCR with different primer ratios; lane M is DNA Marker DL1000; Lanes 1–11 are the primer ratios of 1: 1, 1: 2, 1: 3, 1: 4, 2: 1, 2: 3, 3: 1, 3: 2, 3: 4, 4: 1, 4: 3; Lanes 12–13 are the positive controls for PEDV and PDCoV; and lane 14 is the negative control. (C) The electrophoresis results of SADS-CoV and PDCoV duplex RT-PCR with different primer ratios; lane M is DNA Marker DL1000; Lanes 1–11 are the primer ratios of 1: 1, 1: 2, 1: 3, 1: 4, 2: 1, 2: 3, 3: 1, 3: 2, 3: 4, 4: 1, 4: 3; Lanes 12–13 are the positive controls for PEDV and PDCoV; and lane 14 is the negative control.