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. 2022 Nov 8;9:1033864. doi: 10.3389/fvets.2022.1033864

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Copyright © 2022 Niu, Li, Guan, Deng, Wang, Li, Zhou, Xu, Chen, Zhai and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Primer concentration electrophoresis results of multiplex RT-PCR of TGEV, PEDV, SADS-CoV, and PDCoV. Lane M is DNA Marker DL1000, lanes 1–9 represent the concentration combination of nine primers in the fixed ratio (3:3:3:4) of TGEV, PEDV, SADS-CoV, and PDCoV; lanes 10–13 represent the positive control of TGEV, PEDV, SADS-CoV, and PDCoV; lane 14 was negative control.