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Comparative dose-dependent binding of bhLF to lipid A, Re LPS, and Ra LPS. One hundred microliters of bhLF (twofold dilutions) was added to wells coated with E. coli F 515 lipid A (solid circles), E. coli F 515 Re LPS (open circles), or E. coli EH 100 Ra LPS (solid squares). Binding was evaluated as reported in Materials and Methods. Nonspecific binding was assessed by adding bhLF to wells from which the coating antigen was omitted (open squares).
