TABLE 1.

Inhibition of bhLF binding to solid-phase lipid A and LPS after preincubation with soluble lipid A, BSA, and MAbs AGM 10.14, AGM 2.29, and 16 D7

Molecule	OD at 492 nm after preincubation with the following inhibitor at 2.5 μg/ml:
	Diluent buffera	Lipid Ab	BSA	AGM 10.14	AGM 2.29	16 D7
Lipid A	2.512	0.427	2.499	0.007	2.581	2.470
E. coli Ra LPS	0.560	0.055	0.547	0.012	0.613	0.591
E. coli Re LPS	2.260	0.475	2.222	0.022	2.315	2.198
S. minnesota Ra LPS	0.374	0.087	0.407	0.002	0.364	0.399
S. minnesota Rb LPS	0.382	0.053	0.354	0.018	0.421	0.402
S. minnesota Rc LPS	0.437	0.089	0.415	0.005	0.470	0.451
S. minnesota Rd1 LPS	0.712	0.178	0.687	0.037	0.743	0.698
S. minnesota Rd2 LPS	1.970	0.256	2.001	0.027	2.064	1.952
S. minnesota Re LPS	2.198	0.328	2.144	0.034	2.215	2.067
S. typhi S LPS	0.311	0.047	0.322	0.012	0.341	0.299

^aPreincubation of bhLF (final concentration, 300 ng/ml) with diluent buffer served as a positive control (100% binding). 

^bE. coli F 515 lipid A was used.