TABLE 3.
Internalization of bovine and human S. aureus isolates by MAC-T and adherence to immobilized Fn
| Straina | Percentage of inoculum internalized in:
|
Fn adherence indexd | |
|---|---|---|---|
| Medium alone | Medium + 100 nM Fnb | ||
| 8325-4 | 2.5 ± 0.1 | 0.001 ± 0.001 | 4.3 ± 0.19 |
| DU5883 | 0.1 ± 0.07 | 0.1 ± 0.05 | 0.1 ± 0.01 |
| DU5883(pFnBPA4) | 11.2 ± 0.7 | NDc | 10.8 ± 0.4 |
| Novel | 17.8 ± 1.6 | 1.0 ± 0.04 | 4.0 ± 0.3 |
| 305 | 12.9 ± 0.4 | 0.3 ± 0.02 | 5.3 ± 0.4 |
| Newman | 1.5 ± 0.1 | 0.02 ± 0.006 | 1.3 ± 0.05 |
| MN8 | 18.0 ± 0.8 | 0.1 ± 0.02 | 1.0 ± 0.02 |
| 19095 | 0.5 ± 0.03 | 0.002 ± 0.001 | 0.11 ± 0.01 |
a
Strains are described in Table 1.
b
The purity of commercially obtained bovine Fn was confirmed by analyzing 20 μg of protein per lane of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. The preparation resolved into a homogeneous protein band.
c
ND, not determined.
d
Calculated as described in Materials and Methods.