Table 1.

List of primers used for DKK1 and CD36 knock out and identify.

Primer name	Primer sequence (5′-3′)
L-E2-DKK1-gRNA-F	CACCGTCACGCTATGTGCTGCCCC
L-E2-DKK1-gRNA-R	AAACGGGGCAGCACATAGCGTGAC
R-E2-DKK1-gRNA-F	CACCGCGTTTTCGGCGCTTCCTGC
R-E2-DKK1-gRNA-R	AAACGCAGGAAGCGCCGAAAACGC
E2-DKK1-CHECK-F	GTACCCGGGCGGGAATAAG
E2-DKK1-CHECK-R	ATAGACGCTCAAAGGCTGGAC
L-E1-MDKK1-gRNA-F	CACCGATGATGGTTGTGTGTGCAG
L-E1-MDKK1-gRNA-R	AAACCTGCACACACAACCATCATC
R-E1-MDKK1-gRNA-F	CACCGAGAGCCATCATTGTAAACA
R-E1-MDKK1-gRNA-R	AAACTGTTTACAATGATGGCTCTC
E1-MDKK1-CHECK-F	TTGTTGTCTTCCCTGAGGAGC
E1-MDKK1-CHECK-R	ATCTTCAGCGCAAGGGTAGG
H-L-CD36-gRNA-F	CACCGAGATGGCACCATTGGGCTGC
H-L-CD36-gRNA-R	AAACGCAGCCCAATGGTGCCATCTC
H-R-CD36-gRNA-F	CACCGTTCACTATCAGTTGGAACAG
H-R-CD36-gRNA-R	AAACCTGTTCCAACTGATAGTGAAC
H-CD36-CHECK-F	GCATGCTACCATCTGCCGTA
H-CD36-CHECK-R	TTGCCCACTGGTACAGCTAC
M-L-CD36-gRNA-F	CACCGCCAAAACTGTCTGTACACAG
M-L-CD36-gRNA-R	AAACCTGTGTACAGACAGTTTTGGC
M-R-CD36-gRNA-F	CACCGTGTGCAAAACCCAGATGACG
M-R-CD36-gRNA-R	AAACCGTCATCTGGGTTTTGCACAC
M-CD36-CHECK-F	TTGACTAAGGGAGTGTTGCCA
M-CD36-CHECK-R	GGTCGACTAGGCCATCCTTT