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. 2022 Nov 8;10:1022747. doi: 10.3389/fcell.2022.1022747

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Copyright © 2022 Wang, Shen, Yang and Turan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microscope images (20X) of viral RNA and ACSL4 protein staining in placental villous tissues among specimens with negative PSS staining. RNA-ISH with use of RNAScope HD-RED kit and immunohistochemical staining (IHC) were performed to determine the abundance of the viral RNA genome and ACSL4 protein, respectively.