Subject	Environmental Genomics and Metagenomics
Specific subject area	Metagenomic analysis of freshwater microbes associated with harmful algal blooms
Type of data	Raw Metagenomic Data Metadata Table Figure
How the data were acquired	Freshwater eDNA samples collected from Lake Erie GLERL
Data format	Raw data (fastq.gz.file)
Description of data collection	DNeasy PowerWaterⓇ isolation kit was used to extract DNA from the water samples. DNA was then sequenced using the Oxford Nanopore MinIONⓇ device.
Data source location	Lake Erie NOAA GLERL stations WE13 and WE02. Latitude (approx.) 42.07 N Longitude (approx.) 81.34 W.
Data accessibility	We have deposited the raw sequences in the NCBI Sequence Read Archive (SRA) under accession number PRJNA812770, accessible at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA812770/.