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. 2022 Nov 8;13:887880. doi: 10.3389/fmicb.2022.887880

Table 2.

Percentage of identity matches of 66 fungal isolates from different plant parts of Acacia mangium based on ITS sequences using BLAST analyses, and their percentage of inhibition against Ceratocystis fimbriata.

No. Endophytic isolate ID Plant part Inhibition activities (%) (mean ± standard error) GenBank Accession number ITS region
Match identity (%) E-value Identification in GenBank BLAST match in GenBank Phylum, Class, Family
1 AC 1R Root 55 ± 0.58 MW254902 99.28 0 Blakeslea trispora HQ248186 Zygomycota, Zygomycetes, Choanephoraceae
2 AC 2R Root 0 ± 0.00 MW254903 99.63 0 Trichoderma gamsii KX009501 Ascomycota, Sordariomycetes, Hypocreaceae
3 AC 3R Root 0 ± 0.00 MW254904 100 0 Aspergillus aculeatinus MK281555 Ascomycota, Eurotiomycetes, Trichocomaceae
4 AC 4R Root 44 ± 2.08 MW254905 99.38 0 Nigrospora sphaerica MH368102 Ascomycota, Sordariomycetes, Trichosphaeriales
5 AC 5R Root 20 ± 0.00 MW254913 99.21 0 Aspergillus niger MN474007 Ascomycota, Eurotiomycetes, Trichocomaceae
6 AC 6R Root 8.88 ± 0.66 MW254916 99.63 0 Trichoderma spirale MN227543 Ascomycota, Sordariomycetes, Hypocreaceae
7 AC 7R Root 14.28 ± 0.43 MW254942 99.17 0 Sordariomycetes sp. JQ759985 Ascomycota, Sordariomycetes,
8 AC 8R Root 25 ± 2.89 MW254956 99.58 0 Nigrospora oryzae MN382281 Ascomycota, Sordariomycetes, Trichosphaeriales
9 AC 9R Root 58.33 ± 5.02×1015 MW254964 99.81 0 Trichoderma sp. MK870905 Ascomycota, Sordariomycetes, Hypocreaceae
10 AC 1S Stem 58.33 ± 5.02 ×1015 MW254907 99.81 0 Trichoderma koningiopsis KY807125 Ascomycota, Sordariomycetes, Hypocreaceae
11 AC 2S Stem 33.33 ± 0.29 MW254909 99.79 0 Nigrospora sphaerica KJ572188 Ascomycota, Sordariomycetes, Trichosphaeriales
12 AC 3S Stem 0 ± 0.00 MW254914 99.63 0 Pestalotiopsis vismiae KP747709 Ascomycota, Sordariomycetes, Sporocadaceae
13 AC 4S Stem 0 ± 0.00 MW254920 99.81 0 Pestalotiopsis sp. KY413701 Ascomycota, Sordariomycetes, Sporocadaceae
14 AC 5S Stem 45.45 ± 5.02 ×1015 MW254924 99.45 0 Trichoderma sp. MK870688 Ascomycota, Sordariomycetes, Hypocreaceae
15 AC 6S Stem 20 ± 3.61 MW254925 99.15 0 Lasiodiplodia theobromae GQ502461 Ascomycota, Dothideomycetes, Botryosphaeriaceae
16 AC 7S Stem 45 ± 0.00 MW254931 99.25 0 Trichoderma gamsii KX009501 Ascomycota, Sordariomycetes, Hypocreaceae
17 AC 8S Stem 40 ± 0.00 MW254937 99.59 0 Nigrospora oryzae MN38228 Ascomycota, Sordariomycetes, Trichosphaeriales
18 AC 9S Stem 0 ± 0.00 MW254940 99.63 0 Trichoderma ovalisporum FJ442652 Ascomycota, Sordariomycetes, Hypocreaceae
19 AC 10S Stem 0 ± 0.00 MW254944 99.8 0 Aspergillus niger MN559950 Ascomycota, Eurotiomycetes, Trichocomaceae
20 AC 11S Stem 45.45 ± 2.60 MW254951 100 0 Sordariomycetes sp. KC178665 Ascomycota, Sordariomycetes,
21 AC 12S Stem 14.28 ± 0.30 MW254954 97.98 0 Eutiarosporella sp. KX464132 Ascomycota, Dothideomycetes, Botryosphaeriales
22 AC 13S Stem 0 ± 0.00 MW254959 100 0 Nigrospora sp. MT556677 Ascomycota, Sordariomycetes, Trichosphaeriales
23 AC 1 l Leaf 55.55 ± 5.02 ×1015 MW254906 99.63 0 Trichoderma gamsii KM103313 Ascomycota, Sordariomycetes, Hypocreaceae
24 AC 2 l Leaf 37.5 ± 1.44 MW254908 99.38 0 Nigrospora sphaerica MN625838 Ascomycota, Sordariomycetes, Trichosphaeriales
25 AC 3 l Leaf 45.45 ± 0.75 MW254910 99.81 0 Trichoderma gamsii KX009501 Ascomycota, Sordariomycetes, Hypocreaceae
26 AC 4 l Leaf 16.67 ± 9.53 MW254918 100 0 Curvularia pandanicola MH275056 Ascomycota, Dothideomycetes, Pleosporaceae
27 AC 5 l Leaf 16.67 ± 0.00 MW254919 99.63 0 Pestalotiopsis microspora MT597837 Ascomycota, Sordariomycetes, Sporocadaceae
28 AC 6 l Leaf 45.45 ± 1.16 MW254921 99.81 0 Pestalotiopsis microspora EU137910 Ascomycota, Sordariomycetes, Sporocadaceae
29 AC 7 l Leaf 58.3 ± 5.02 ×1015 MW254922 98.77 0 Nigrospora oryzae MN382281 Ascomycota, Sordariomycetes, Trichosphaeriales
30 AC 8 l Leaf 28.57 ± 2.51 ×1015 MW254923 99.58 0 Fusarium chlamydosporum MT448890 Ascomycota, Sordariomycetes, Nectriaceae
31 AC 9 l Leaf 0 ± 0.00 MW254926 99.38 0 Nigrospora sphaerica MN566004 Ascomycota, Sordariomycetes, Trichosphaeriales
32 AC 10 l Leaf 30 ± 5.77 MW254934 99.57 0 Lasiodiplodia theobromae KF293981 Ascomycota, Dothideomycetes, Botryosphaeriaceae
33 AC 11 l Leaf 12.5 ± 6.93 MW254936 99.63 0 Trichoderma koningiopsis JQ617301 Ascomycota, Sordariomycetes, Hypocreaceae
34 AC 12 l Leaf 0 ± 0.00 MW254938 99.44 0 Pestalotiopsis neglecta MN006391 Ascomycota, Sordariomycetes, Sporocadaceae
35 AC 13 l Leaf 22.22 ± 0.00 MW254939 99.62 0 Trichoderma gamsii KX009501 Ascomycota, Sordariomycetes, Hypocreaceae
36 AC 14 l Leaf 0 ± 0.00 MW254943 99.58 0 Nigrospora oryzae JX966549 Ascomycota, Sordariomycetes, Trichosphaeriales
37 AC 15 l Leaf 33.33 ± 1.59 MW254945 99.79 0 Nigrospora sp. MT561433 Ascomycota, Sordariomycetes, Trichosphaeriales
38 AC 16 l Leaf 45.45 ± 5.02 ×1015 MW254946 99.58 0 Lasiodiplodia theobromae MK696043 Ascomycota, Dothideomycetes, Botryosphaeriaceae
39 AC 17 l Leaf 40 ± 5.77 MW254948 99.43 0 Pestalotiopsis vismiae KP747709 Ascomycota, Sordariomycetes, Sporocadaceae
40 AC 18 l Leaf 25 ± 0.00 MW254949 99.59 0 Nigrospora sphaerica MT043797 Ascomycota, Sordariomycetes, Trichosphaeriales
41 AC 19 l Leaf 0 ± 0.00 MW254950 99.8 0 Aspergillus aculeatus KJ605160 Ascomycota, Eurotiomycetes, Trichocomaceae
42 AC 20 l Leaf 40 ± 5.77 MW254962 99.59 0 Nigrospora sphaerica MH368102 Ascomycota, Sordariomycetes, Trichosphaeriales
43 AC 21 l Leaf 0 ± 0.00 MW254963 99.58 0 Nigrospora sphaerica MT561433 Ascomycota, Sordariomycetes, Trichosphaeriales
44 AC 1P Petiole 0 ± 0.00 MW254917 99.81 0 Trichoderma crissum MK911703 Ascomycota, Sordariomycetes, Hypocreaceae
45 AC 2P Petiole 0 ± 0.00 MW254932 99.79 0 Nigrospora sphaerica MT561433 Ascomycota, Sordariomycetes, Trichosphaeriales
46 AC 3P Petiole 50 ± 4.91 MW254933 97.68 0 Nigrospora sphaerica MN795570 Ascomycota, Sordariomycetes, Trichosphaeriales
47 AC 4P Petiole 58.33 ± 5.02 ×1015 MW254935 99.38 0 Nigrospora sphaerica KJ572188 Ascomycota, Sordariomycetes, Trichosphaeriales
48 AC 5P Petiole 45.45 ± 5.02 ×1015 MW254947 99.79 0 Nigrospora sphaerica MT561433 Ascomycota, Sordariomycetes, Trichosphaeriales
49 AC 6P Petiole 45 ± 5.77 MW254952 99.8 0 Penicillium rolfsii MK120600 Ascomycota, Eurotiomycetes, Trichocomaceae
50 AC 7P Petiole 20 ± 5.77 MW254957 100 0 Trichoderma longibrachiatum FJ462745 Ascomycota, Sordariomycetes, Hypocreaceae
51 AC 8P Petiole 30 ± 5.77 MW254958 99.37 0 Neopestalotiopsis cubana LC521857 Ascomycota, Sordariomycetes, Pestalotiopsidaceae
52 AC 9P Petiole 45.45 ± 5.02 ×1015 MW254961 99.79 0 Pestalotiopsis sp. JN116590 Ascomycota, Sordariomycetes, Sporocadaceae
53 AC 10P Petiole 45.45 ± 5.02 ×1015 MW254965 99.57 0 Lasiodiplodia theobromae MT075441 Ascomycota, Dothideomycetes, Botryosphaeriaceae
54 AC 11P Petiole 14.28 ± 0 MW254966 70.1 4.00E-20 Trichoderma sp. GU973813 Ascomycota, Sordariomycetes, Hypocreaceae
55 AC 12P Petiole 20 ± 5.77 MW254967 99.44 0 Trichoderma koningiopsis MT102395 Ascomycota, Sordariomycetes, Hypocreaceae
56 AC 1F Flower 8.88 ± 0.00 MW254911 99.28 0 Blakeslea trispora HQ248186 Zygomycota, Zygomycetes, Choanephoraceae
57 AC 2F Flower 8.88 ± 5.14 MW254915 94.87 0 Hypoxylon monticulosum KY610404 Ascomycota, Sordariomycetes, Hypoxylaceae
58 AC 3F Flower 58.33 ± 5.02 ×1015 MW254927 100 0 Nigrospora sphaerica MT561433 Ascomycota, Sordariomycetes, Trichosphaeriales
59 AC 4F Flower 40 ± 0.00 MW254941 99.62 0 Trichoderma longibrachiatum MH745146 Ascomycota, Sordariomycetes, Hypocreaceae
60 AC 5F Flower 45.45 ± 2.60 MW254953 99.59 0 Pseudopestalotiopsis theae KX401429 Ascomycota, Sordariomycetes, Pestalotiopsidaceae
61 AC 6F Flower 45.45 ± 5.02 ×1015 MW254955 100 0 Trichoderma koningiopsis JQ278013 Ascomycota, Sordariomycetes, Hypocreaceae
62 AC 1 U Fruit 8.88 ± 0.00 MW254912 100 0 Pestalotiopsis microspore EU137910 Ascomycota, Sordariomycetes, Sporocadaceae
63 AC 2 U Fruit 69.23 ± 0.00 MW254928 99.79 0 Lasiodiplodia theobromae MK696044 Ascomycota, Dothideomycetes, Botryosphaeriaceae
64 AC 3 U Fruit 25 ± 2.89 MW254929 98.39 0 Blakeslea trispora HQ248186 Zygomycota, Zygomycetes, Choanephoraceae
65 AC 4 U Fruit 45 ± 2.89 MW254930 99.79 0 Lasiodiplodia venezuelensis MH865369 Ascomycota, Dothideomycetes, Botryosphaeriaceae
66 AC 5 U Fruit 16.16 ± 0.00 MW254960 100 0 Trichoderma harzianum MF537642 Ascomycota, Sordariomycetes, Hypocreaceae