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. 2022 Oct 11;10:988699. doi: 10.3389/fcell.2022.988699

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Copyright © 2022 Monferrer, Dobre, Trujillo, González Oliva, Trubert-Paneli, Acevedo-León, Noguera and Salmeron-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell viability in 3D VN based hydrogels. (A). Images of calcein-AM (green) and ethidium homodimer-1 (red) for SK-N-BE (2) in 3 and 10 wt% VN/PEG with 500 μg ml⁻¹ of VN. (B). Quantification of SK-N-BE (2) viability with ANOVA statistical method. (C). Cell cluster area for 3 and 10 wt% VN/PEG hydrogels after 1, 5, and 7 days (scale bar 50 µm). Statistical differences were analyzed by Kruskal–Wallis test. (D). Mean number of clusters per field area for 3 and 10 wt% VN/PEG hydrogels, grouping images from 1, 5 and 7 days together. Mann-Whitney analysis was performed to compare the number of clusters. A total of 15 images (3 replicates, 5 images each) were taken per sample condition.