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. 2022 Oct 11;10:988699. doi: 10.3389/fcell.2022.988699

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Copyright © 2022 Monferrer, Dobre, Trujillo, González Oliva, Trubert-Paneli, Acevedo-León, Noguera and Salmeron-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of VN secreted by the cells in 3D PEG and VN/PEG hydrogels. Immunofluorescence images of SK-N-BE (2) cultured for 7 days in 3 and 10 wt% PEG and VN/PEG. Images show nuclei (blue) and VN (magenta) (scale bar 50 µm). (B). Immunofluorescence quantification of VN secreted by cells in 3 and 10 wt% PEG with/without VN. We have represented in the graph the Integrated density (total raw intensity per image normalized by cell area). A total of 15 images (3 replicates, 5 images each) were taken per sample condition.