Figure 1.

Glycan microarray data sets generated with the Consortium for Functional Glycomics glycan microarray version 5 (CFGv5) containing 611 unique glycans. Biotinylated lectins (116 total) were incubated with the arrays at varying concentrations, followed by incubation with Cy5-Streptavidin. Slides were processed and scanned, and data were extracted. For each array, the average fluorescence for each glycan (n = 4 spots) was obtained. Array data sets were then used as input for machine learning and Z-score analysis to identify motifs.