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GusA reporter available to evaluate different promoters in E. coli and Clostridium. (a) Specific activities of glucuronidase of negative controls pGG2121 and pGG2121-gusA in E. coligusA–, C. sporogenes-NT, and C. butyricum. (b) Strengths of native promoters (Pthl, Pfdx, Pptb, ParaE), promoter_tU, and promoter_fU fusions in E. coligusA– and C. sporogenes-NT by glucuronidase assay. Data represent the mean ± s.d. of three biological replicates.
