Table 1. Lactate Production in Z. mobilis ZM4 Using Different Inducible and Constitutive Promotors^a.

strain	promoter	inducer	titer [mM lactate]	lactate yield [mol/mol glucose]
WT			<1	0
pZP537 empty vector	Pstrong100k*	constitutive	<1	0
pZP561	PA1lacO1	uninduced	19.6 ± 0.6	0.09
pZP561	PA1lacO1	10 μM IPTG	42.3 ± 4.8	0.19
pZP561	PA1lacO1	100 μM IPTG	70.6 ± 3.7	0.33
pZP561	PA1lacO1	500 μM IPTG	85.2 ± 2.4	0.40
pZP561	PA1lacO1	1 mM IPTG	83.1 ± 0.9	0.38
pZP255	Ptet	216 nM aTc	35.0 ± 1.7	0.17
pZP255	Ptet	500 nM aTc	29.0 ± 1.9	0.14
pZP374	PlacUV5	constitutive	81.0 ± 2.6	0.38
pZP536	Pstrong100k*	constitutive	86.0 ± 4.0	0.41
ΔZMO0028::ldhA	Pstrong100k*	constitutive	43.1 ± 0.6	0.21

^aThe detection limit of lactate is about 1 mM.