Table 1.
Primers used in this paper.
| Primers | Sequences (5′−3′) |
|---|---|
| Quantitative PCR | |
| LvBigPEN-F | ACCACAGACCCCAAGTCCTA |
| LvBigPEN-R | AGTTCCGGCAGATTTCGGTT |
| c-Fos-F | CCATTACAGCTGTGGCTACGAGT |
| c-Fos-R | GGTCTGTTCGATGTTCCTCAAG |
| c-Jun-F | GACGCCCTCCCAGTTCTTCTT |
| c-Jun-R | CTGGTGGAGATGGCATCCTG |
| V. parahemolyticus - | |
| 16s-F | GGTGTAGCGGTGAAATGCGTAG |
| V. parahemolyticus - | |
| 16s-R | CCACAACCTCCAAGTAGACATCG |
| EF-1α-F | TATGCTCCTTTTGGACGTTTTGC |
| EF-1α-R | CCTTTTCTGCGGCCTTGGTAG |
| Protein expression | |
| LvBigPEN-F | GAGGGGCCGCCTGGAGTGCTGCGTCCTC |
| LvBigPEN-R | ACAGCAGGAGTTCCAGCGCTTGCAG |
| RNA interference | |
| T7-LvBigPEN-F | GGATCCTAATACGACTCACTATAGGGCCTATTGCTCGCCCTCA |
| T7-LvBigPEN-R | GGATCCTAATACGACTCACTATAGGCGGCAGATTTCGGTTTCC |
| T7-c-Fos-F | GGATCCTAATACGACTCACTATAGGCCCACTTCGTCCTCGTCTTCG |
| T7-c-Fos-R | GGATCCTAATACGACTCACTATAGGAGGTCCTCCTCGTGCTCCAT |
| T7-c-Jun-F | GGATCCTAATACGACTCACTATAGGACCATCCTCAACAGCAACACG |
| T7-c-Jun-R | GGATCCTAATACGACTCACTATAGGCGCTCCTGGCACTCCATATC |
| T7-GFP-F | GGATCCTAATACGACTCACTATAGGATGGTGAGCAAGGGCGAGGAG |
| T7-GFP-R | GGATCCTAATACGACTCACTATAGGTTACTTGTACAGCTCGTCCATGCC |
| Dual-luciferase | |
| pGL3-LvBigPEN-F | GGGGTACCCACATACACACACATATATATATGTGTCTGTC |
| pGL3-LvBigPEN-R | CCGCTCGAG GTCTGTCCTCTTCGTGCTGATCAAG |