Table 3.

Key validation data for the two multiplex real-time PCR assays. nd = not determined.

Species	Multiplex	Average E ± SD	Average R2	LODasym
Paramoeba perurans	1	95.1 ± 2.3	0.998	15
Desmozoon lepeophtherii	1	101.6 ± 2.5	0.996	5
Ca. Branchiomonas cysticola	2	87.7 ± 3.2	0.998	5
Salmon gill poxvirus	2	90.5 ± 3.1	0.996	7
Salmo salar	1	93.4 ± 1.6	0.997	nd
Salmo salar	2	89.3 ± 2.2	0.995	nd