Fig. 2.

Specific binding of SGRPs to sialoglycans in mammalian sera. The figure presents SGRPs binding of sialoglycans in biological samples, here represented by mammalian sera from mouse, rat, rabbit, Guinea pig, goat, sheep, horse, human, and Cmah^−/− mouse. Panels A–I (except panel H) show binding efficiency of SGRPs and their nonbinding controls toward sera in concentration-dependent manner, as marked in individual panels. Panel H shows binding of SGRP8^TeT and SGRP8^TeTNB of purified gangliosides at concentrations mentioned in panel. Panel J demonstrates the 7,9-diOAc-Sia-specific binding of SGRP7^BCoV with bovine submaxillary mucin, while its mutant SGRP7^BCoVNB shows no affinity toward BSM. Binding of biotinylated SGRPs was detected and developed using Avidin-HRP (1:1,500). ELISA for each SGRP pair was performed under the same experimental conditions and validity of binding was confirmed by background signal wells treated only with secondary antibody (Avidin-HRP), not shown here.