Table 1.
Sialoglycan recognition probes and their specificities.
| Class of SialoGlycan Recognition Probe | Sialoglycans classes | Identified molecule | Pfam IDs | Sialoglycan preferences | SGRPs Nomenclature | |
|---|---|---|---|---|---|---|
| Binding | Non-binding | |||||
| SGRP1 | All Sialic Acids (Sias) | YenB | Pertussis_S2S3 (PF02918) | All Sias | SGRP1YenB | SGRP1YenBNB |
| SGRP2 | 5-N-acetylneuraminic acid (Neu5Ac) | PltB | Pertussis_S2S3 (PF02918) | All Neu5Ac glycans | SGRP2PltB | SGRP2PltBNB |
| SGRP3 | α-2-3 Linked Sialic Acids (α-2-3 Sias) | HsaBR | GspA_SrpA_N (PF20164) | All α2–3-linked Sias | SGRP3Hsa | SGRP3HsaNB |
| SGRP4 | 4-O-Acetylated Sialic Acids (4-OAc Sias) | MHV | Hema_esterase (PF03996) | 4-OAcetylated Neu5Ac | SGRP4MHV | SGRP4MHVNB |
| SGRP5 | 5-N- glycolylneuraminic acid (Neu5Gc) | IgY | Ig (PF00047) | All Neu5Gc glycans | SGRP5IgY | SGRP5IgYNB |
| SGRP6 | α-2-6 Linked Sialic Acids (α-2-6 Sias) | SNA | Ricin_B_lectin (PF00652) | All α2–6 Linked Sia | SGRP6SNA | N/A |
| SGRP7 | 7-O-Acetylated Sialic Acids (7-OAc Sias) | BCoV | Hema_esterase (PF03996) | 7–9-di O-Acetylated Sia | SGRP7BCoV | SGRP7BCoVNB |
| SGRP8 | α-2-8 Linked Di-Sialic Acid linkage | TeT | Toxin_R_bind_C (PF07951) | Disialic linkages | SGRP8TeT | SGRP8TeTNB |
| SGRP9 | 9-O-Acetylated Sialic Acids (9-OAc Sias) | PToV | Hema_esterase (PF03996) | All 9-O-Acetylated Sia | SGRP9PToV | SGRP9PToVNB |
Table representing the classes of sialoglycan recognition probes (SGRPs), their presumed binding specificities, the most appropriate molecules as observed by assessment of sialoglycan binding by various glycomic methods, their experimentally confirmed sialoglycan preferences and defining SGRP nomenclature suggesting class of probe along with source of probe as mentioned in superscripts. The names of probes ending with NB represent the nonbinding variant of SGRPs.