FIG 8.
VdCf2 regulated the expression of a putative secondary metabolism gene cluster. (A) Visualization of RNA-seq coverage of this putative secondary metabolism gene cluster in the wild-type and ΔVdCf2 strains. The blue lines indicate read coverage. (B) Gene expression level of the Vd276-280 gene cluster. The conidial suspensions (107 conidia/mL) were cultured in the Czapek-Dox medium for 5 days before being treated with cotton root extracts for 2 days. The β-tubulin gene (VDAG_10074) was used as the internal reference. The expression level of each gene in the wild-type strain in the control was standardized as 1. Error bars represent standard errors calculated from three replicates, and different letters indicate the statistical significance of Tukey’s test at a P value of 0.05.