FIG 3.

Differences in density between wAlbB variants. (A and B) Female (A) and male (B) Wolbachia density in reciprocally backcrossed Aedes aegypti populations. Populations have different combinations of Wolbachia infection type/mitochondrial haplotype (wAlbB-Hou and Au or wAlbB-Uju and My) and nuclear background (Au or My). Data from two replicate populations were pooled for visualization. (C and D) Wolbachia density in (C) females and (D) males following exposure to cyclical heat stress during the egg stage. Eggs were exposed to cyclical temperatures of 29 to 39°C for 7 days (red circles) or held at 26°C (black circles). Each point represents the relative density for an individual averaged across 2 to 3 technical replicates. Medians and 95% confidence intervals are shown in black lines. Data for wAlbB-I and wMel have also been included from reference 44.