FIG 3.

(A) Metabolic diagram of glycolysis around the PFK reaction and the enzymes that catalyze the reactions. (B) ¹³C-labeling patterns for key glycolytic metabolites (glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, and dihydroxyacetone phosphate) for cells fed a 50:50 mixture of uniformly labeled and naturally labeled glucose. The “M+” notation indicates the number of ¹³C-labeled carbon atoms (i.e., M+6 indicates six labeled carbon atoms). The subscript after the strain ID number indicates biological replicate number. The cofactor specificity of the PFK reaction is indicated below the strain name: “+” indicates the presence of a reaction, and “−” indicates the absence of a reaction.