TABLE 2.
Plasmids used in this study
| Plasmid | Description | Accession no. | Source or reference |
|---|---|---|---|
| pDGO143 | C. thermocellum expression vector | KX259110 | 26 |
| pLL1381 | pDGO143 with C. thermocellum Clo1313_2638 promoter driving T. saccharolyticum ATP-pfk; native RBS with predicted translation initiation rate of ~95,000 arbitrary units | ON809502 | This study |
| pLL1382 | pDGO143 with C. thermocellum Clo1313_2638 promoter driving T. saccharolyticum ATP-pfk; modified RBS with predicted translation initiation rate of ~500 arbitrary units | ON809503 | This study |
| pLL1383 | pDGO143 with T. saccharolyticum ATP-pfk; no promoter driving ATP-pfk expression | ON809504 | This study |
| pLL1384 | pDGO143 with C. thermocellum Clo1313_2638 promoter driving T. saccharolyticum ATP-pfk; modified RBS with predicted translation initiation rate of ~4,000 arbitrary units | ON809505 | This study |
| pLL1385 | pDGO143 with C. thermocellum Clo1313_2638 promoter driving T. saccharolyticum ATP-pfk; modified RBS with predicted translation initiation rate of ~8,000 arbitrary units | ON809506 | This study |
| pLL1386 | pDGO143 with C. thermocellum Clo1313_2638 promoter driving T. saccharolyticum ATP-pfk; modified RBS with predicted translation initiation rate of ~14,000 arbitrary units | ON809507 | This study |
| pLL1387 | pDGO143 with C. thermocellum Clo1313_2638 promoter driving T. saccharolyticum ATP-pfk; modified RBS with predicted translation initiation rate of ~27,000 arbitrary units | ON809508 | This study |
| pLL1388 | Integration vector; introduces T. saccharolyticum transaldolase and its native promoter immediately downstream of C. thermocellum PPi-pfk gene | ON809509 | This study |
| pLL1389 | Integration vector; introduces T. saccharolyticum ATP-phosphofructokinase downstream of Clo1313_0718 (putative ADP-glucose synthase), with a spacer sequence between Clo1313_0718 and the ATP-pfk | ON809510 | This study |
| pLL1390 | Replacement vector; deletes Clo1313_0717-0718 and the spacer sequence, and replaces it with the T. saccharolyticum ATP-pfk promoter sequence | ON809511 | This study |
| pLL1391 | Deletion vector; deletes C. thermocellum PPi-pfk. In strain LL1647 and its derivatives, this plasmid also deletes the previously introduced T. saccharolyticum transaldolase | ON809512 | This study |
| pLL1392 | Deletion vector; deletes C. thermocellum PPi-pfk only in strain LL1647 and its derivatives | ON809513 | This study |